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KMID : 1004820110120010023
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Journal of Biomedical Research
2011 Volume.12 No. 1 p.23 ~ p.33
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Neuroprotective Effect of Resveratrol against Glutamate-induced Toxicity in Mouse Cerebral Cortical Neurons
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Lee Jong-Geol
Yon Jung-Min
Lin Chunmei
Jung A-Young
Jung Ki-Youn
Lee Beom-Jun
Yun Young-Won
Nam Sang-Yoon
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Abstract
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Neurotoxicity and oxidative injury induced by glutamate cause neuronal degeneration related to various central nervous system diseases. Resveratrol, a polyphenolic compound, is known to have antioxidative and anti-inflammatory effects. The aim of this study was to investigate the question of whether resveratrol has a neuroprotective effect against glutamate-induced toxicity in cultured cortical neurons. Following exposure to glutamate for 15 min, cortical neurons originating from ICR mouse fetuses on embryonic days 15-16 were then treated with resveratrol for 24 h in the post-treatment paradigm. Glutamate induced a significant reduction in cell viability; however, resveratrol induced a significant increase in cell viability. Glutamate induced generation of ROS and apoptotic neuronal death; however, these were decreased by exposure to resveratrol. mRNA expression in antioxidant enzymes, cytoplasmic glutathione peroxidase, copper/zinc superoxide dismutase (SOD), and manganese SOD, and anti-apoptotic regulator Bcl-xL were decreased by exposure to glutamate, however, exposure to resveratrol resulted in a significant increase in their mRNA levels. In addition, mRNA expression of pro-inflammatory cytokines, interleukin-1β and tumor necosis factor-α, was increased by glutamate insult, but significantly reduced by resveratrol. These findings indicate that resveratrol is neuroprotective against glutamate-induced toxicity, suggesting a useful therapeutic application in treatment of neurodegenerative disorders.
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KEYWORD
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Glutamate, Resveratrol, Neuroprotection, Cortical neuron
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